High Pressure Liquid Chromatographic Techniques for the Separation of Complex Mixtures of Occurring Porphyrins Naturally
Carlson, R.E. and David Dolphin
"Porphyrins in Human Diseases," M. Doss, Ed., Karger, Basel
Porphyrins can be rapidly and exhaustively isolated from urine via florisil chromatography and diazomethane esterification. Separation of the isolated porphyrins via HPLC provides a rapid qualitative and quantitative analysis of the sample. Using these techniques, a urine sample, in which the porphyrinogens have been oxidized, can be analyzed within 1 h.