Ligand Binding to Cytochrome P450cam: Characteristics of the Reduced Species
David Dolphin, James, B.R., and Welborn, C.
Microsomes, Drug Oxidations, and Chemical Carcinogenesis Academic Press, New York,
An analysis of spectral and ligation data for native Pseudomonas putida P 450cam and for four synthetic heme derivatives of P 450cam is presented. This analysis emphasizes the unique characteristics of the thiolate-ligated heme. A simple purification method for heme-substituted P 450cam is also described.