Pub No:
116
Title:
Preparation of Ruthenium(II) and RutheČnium(III) Myoglobin and the Reaction of Dioxygen, and Carbon Monoxide, with Ruthenium(II) Myoglobin
Authors:
Paulson, D.R., Addison, A.W., David Dolphin, and James, B.R
Journal:
J. Biol. Chem.
Year:
1979
Pages:
254, 7002-7006
Abstract:
Ru myoglobins were prepared by the reconstitution of horse heart apomyoglobin with either Ru(II) or Ru(III) mesoporphyrin IX (MpIX) derivatives. The Ru(II) and -(III) myoglobins (RuMb and RuMb+) contained 1 Ru porphyrin/heme binding site; the species were readily interconverted using dithionite for reduction and Br2 for oxidation. RuMb bound CO to give the known carbonyl complex. Reversible oxygenation occurred readily with protein-free Ru(II)(MpIX) species in DMF but RuMb in phosphate buffer was irreversibly oxidized by O2 to give RuMb+ via an outer sphere electron transfer mechanism.

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