PATENT DETAILS

Patent ID:
193
Inventors:
David Dolphin, Xuejun Peng, Ethan Sternberg
Title:
Chiral Separation Of Benzoporphyrin Derivative Mono- And Di-Acids By Laser-Induced Fluorescence Capillary Electrophoresis
Country:
United States
Patent No:
6878253
Grant Date:
2005-04-12
Abstract:
A method for the separation of benzoporphyrin derivative mono and diacid (BPD-MA, BPD-DA) enantiomers by Laser-Induced Fluorescence Capillary Electrophoresis has been developed. The limits of detection are 2.0610?6 M, and the relative standard deviation for the separation was 2.90% to 4.64%. The BPD enantiomers can be quantitatively determined in the range of 10?2 to 10?5 mg mL?1. In comparison with HPLC, CE has better resolution and efficiency. This separation method was successfully applied to the BPD enantiomers obtained from a matrix of bovine serum and from liposomally formulated material as well as from studies with rat, dog and human microsomes.

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